Lactic dehydrogenase and cytochrome b2 of baker's yeast; purification and crystallization.
نویسندگان
چکیده
Ammon, R. & Savelsberg, W. (1949). Hoppe-Seyl. Z. 284, 135. Augustinsson, K.-B. (1948). Acta physiol. scand. 15, suppl. 52. Augustinsson, K.-B. (1957). Meth. biochem. Anal. 5, 1. Augustinsson, K.-B. (1958a). Nature, Lond., 181, 1786. Augustinsson, K.-B. (1958b). Acta chem. 8cand. 12, 1150. Augustinsson, K.-B. & Olsson, B. (1959). Biochem. J. 71,477. Bailie, M. J. & Morton, R. K. (1958). Biochem. J. 69, 35. Deutsch, H. F. (1947). J. biol. Chem. 169, 437. Foster, J. F., Friedell, R. W., Catron, D. & Dieckmann, M. R. (1951). Arch. Biochem. Biophy8. 31, 104. Glick, D., Levin, A. & Antopol, W. (1939). Proc. Soc. exp. Biol., N. Y., 40, 28. Hines, B. E. & McCance, R. A. (1953). J. Physiol. 122, 188. Hoerlein, A. B. (1957). J. Immunol. 78, 112. Kalow, W. & Staron, N. (1957). Canad. J. Biochem. Physiol. 35, 1305. McCance, R. A., Brown, L. M., Comline, R. S. & Titchen, D. A. (1951). Nature, Lond., 168, 788. Mathews, J. & Buthala, D. A. (1956). Amer. J. vet. Re8. 17, 485. Nordbring, F. (1957). Acta Soc. Med. Up8alien. 62, 135. Nordbring, F. & Olsson, B. (1957). Acta Soc. Med. Upsalien. 62, 193. Sawin, P. B. & Glick, D. (1943). Proc. nat. Acad. Sci., Wash., 29, 55. Staub, H. & Boguth, W. (1956). Z. Veterindrmed. 3, 653. Wagner, R. P. & Mitchell, H. K. (1955). Genetics and Metabolism, p. 162. New York: John Wiley and Sons, Inc.
منابع مشابه
Isolation and characterization of the cytochrome domain of flavocytochrome b2 expressed independently in Escherichia coli.
The cytochrome domain of flavocytochrome b2 (L-lactate dehydrogenase) was expressed in the bacterium Escherichia coli and a purification procedure was developed. When expressed in E. coli, the b2-cytochrome domain contains protohaem IX and has an electronic absorption spectrum identical with that of the cytochrome b2 'core' produced by proteolytic cleavage of the enzyme isolated from yeast. The...
متن کاملThe Lactic Dehydrogenases of Yeast
Until about 1957 the L(+)-lactic dehydrogenase of aerobic cells was the only enzyme concerned with lactate oxidation known to occur in yeast. At that time, essentially simultaneously and independently, four laboratories discovered the existence of a second lactic dehydrogenase in yeast, which, in distinction to the L enzyme, occurred in anaerobic cells and reacted with ferricyanide but not cyto...
متن کاملThe lactic dehydrogenase of Penicillium chrysogenum.
A soluble, cytochrome linked flavoprotein which oxidizes lactic acid to pyruvic acid has been isolated from Penicillium chrysogenum, strain NRRL 1951 -B25. The reduction of cytochrome c by a flavin enzyme has precedence in the work of Horecker and Heppel (1949) and Mlorell (1952) who have shown that xanthine oxidase can reduce cytochrome c. Morell reported the enzyme to be inhibited strongly by...
متن کاملInvolvement of a B-type cytochrome in the assimilatory nitrate reductase of Neurospora crassa.
The enzyme systems which catalyze the reduction of nitrate to nitrite have generally been classified into two broad types: (a) assimilatory nitrate reductase, which is responsible for the first step in the reduction of nitrate for the ultimate biosynthesis of nitrogen-containing cell constituents (e.g., amino acids aind purines and pyrimidines), and (b) respiratory or dissimilatory nitrate redu...
متن کاملInduction of D(-)- and L-lactic cytochrome c reductase in yeast.
Aerobically grown bakers’ yeast has been shown to contain two different lactic cytochrome c reductases, one of which is specific for L( +)-lactate (1) and the other of which is specific for D( -)-lactate (2). Neither of these two enzymes is present in anaerobically grown yeast, which contains instead a D( -)lactic dehydrogenase that does not reduce cytochrome c. No L( +)-lactic dehydrogenase is...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- The Biochemical journal
دوره 71 3 شماره
صفحات -
تاریخ انتشار 1959